Part:BBa_K1918103
TRE-Kozak-EBFP2N(1-154):IntN
This part is consist of EBFP2N(1-154):IntN and the TRE promoter. EBFP2N(1-154):IntN is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2 and IntN. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2N(1-154):IntN could be induced by Dox.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 379
Illegal XbaI site found at 1123 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 379
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 379
Illegal BamHI site found at 362
Illegal XhoI site found at 19 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 379
Illegal XbaI site found at 1123 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 379
Illegal XbaI site found at 1123 - 1000COMPATIBLE WITH RFC[1000]
In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.
Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.
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